abstract：:Janus kinases (JAKs) are a family of cytoplasmatic tyrosine kinases that are attractive targets for the development of anti-inflammatory drugs given their roles in cytokine signaling. One question regarding JAKs and their inhibitors that remains under intensive debate is whether JAK inhibitors should be isoform select...

journal_title：Cell chemical biology

authors： Forster M,Chaikuad A,Bauer SM,Holstein J,Robers MB,Corona CR,Gehringer M,Pfaffenrot E,Ghoreschi K,Knapp S,Laufer SA

更新日期：2016-11-17 00:00:00

abstract：:Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM views of multiple subcellular components. The method uses sequential,...

journal_title：Cell chemical biology

authors： Adams SR,Mackey MR,Ramachandra R,Palida Lemieux SF,Steinbach P,Bushong EA,Butko MT,Giepmans BNG,Ellisman MH,Tsien RY

更新日期：2016-11-17 00:00:00

abstract：:G-quadruplexes are specialized secondary structures in nucleic acids that possess significant conformational polymorphisms. The precise G-quadruplex conformations in vivo and their relevance to biological functions remain controversial and unclear, especially for telomeric G-quadruplexes. Here, we report a novel singl...

journal_title：Cell chemical biology

authors： Liu HY,Zhao Q,Zhang TP,Wu Y,Xiong YX,Wang SK,Ge YL,He JH,Lv P,Ou TM,Tan JH,Li D,Gu LQ,Ren J,Zhao Y,Huang ZS

更新日期：2016-10-20 00:00:00

abstract：:Phenotypic drug discovery offers some advantages over target-based methods, mainly because it allows drug leads to be tested in systems that more closely model distinct disease states. However, a potential disadvantage is the difficulty of linking the observed phenotype to a specific cellular target. To address this p...

journal_title：Cell chemical biology

authors： Liu X,Baarsma HA,Thiam CH,Montrone C,Brauner B,Fobo G,Heier JS,Duscha S,Königshoff M,Angeli V,Ruepp A,Campillos M

更新日期：2016-10-20 00:00:00

abstract：:Protein kinases are attractive therapeutic targets because their dysregulation underlies many diseases, including cancer. The high conservation of the kinase domain and the evolution of drug resistance, however, pose major challenges to the development of specific kinase inhibitors. We recently discovered selective Sr...

journal_title：Cell chemical biology

authors： Aleem S,Georghiou G,Kleiner RE,Guja K,Craddock BP,Lyczek A,Chan AI,Garcia-Diaz M,Miller WT,Liu DR,Seeliger MA

更新日期：2016-09-22 00:00:00

abstract：:Mycobacterium tuberculosis synthesizes a wide variety of complex lipids that can serve as antigens in immune recognition of the bacterium. In this issue of Cell Chemical Biology, Gilleron et al. (2016) identify key enzymes essential for lipid antigen processing, which is required for CD1b-restricted T cell activation....

journal_title：Cell chemical biology

authors： Sander P,Becker K,Molin MD

更新日期：2016-09-22 00:00:00

abstract：:Anti-apoptotic BCL-2 family proteins block cell death by trapping the critical α-helical BH3 domains of pro-apoptotic members in a surface groove. Cancer cells hijack this survival mechanism by overexpressing a spectrum of anti-apoptotic members, mounting formidable apoptotic blockades that resist chemotherapeutic tre...

journal_title：Cell chemical biology

authors： Huhn AJ,Guerra RM,Harvey EP,Bird GH,Walensky LD

更新日期：2016-09-22 00:00:00

abstract：:In the nematode Caenorhabditis elegans, inactivating mutations in the insulin/IGF-1 receptor, DAF-2, result in a 2-fold increase in lifespan mediated by DAF-16, a FOXO-family transcription factor. Downstream protein activities that directly regulate longevity during impaired insulin/IGF-1 signaling (IIS) are poorly ch...

journal_title：Cell chemical biology

authors： Martell J,Seo Y,Bak DW,Kingsley SF,Tissenbaum HA,Weerapana E

更新日期：2016-08-18 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) is a target of signal-derived H2O2, and oxidation of active-site cysteine 797 to sulfenic acid enhances kinase activity. Although a major class of covalent drugs targets C797, nothing is known about its catalytic importance or how S-sulfenylation leads to activation. Here, we re...

journal_title：Cell chemical biology

authors： Truong TH,Ung PM,Palde PB,Paulsen CE,Schlessinger A,Carroll KS

更新日期：2016-07-21 00:00:00

abstract：:Energy coupling factor (ECF) transporters are responsible for the uptake of essential scarce nutrients in prokaryotes. This ATP-binding cassette transporter family comprises two subgroups that share a common architecture forming a tripartite membrane protein complex consisting of a translocation component and ATP hydr...

journal_title：Cell chemical biology

authors： Josts I,Almeida Hernandez Y,Andreeva A,Tidow H

更新日期：2016-07-21 00:00:00

abstract：:Owing to their structural flexibility, most serpins inhibit the cognate proteases in a fast and specific manner and also are susceptible to pathogenic misfolding. In this issue of Cell Chemical Biology, Madsen et al. (2016) report on the selection and characterization of an RNA aptamer that stabilizes α1-antichymotryp...

journal_title：Cell chemical biology

authors： Zhou X,Declerck PJ

更新日期：2016-06-23 00:00:00

abstract：:Proper functioning of organelles such as the ER or the Golgi apparatus requires luminal accumulation of Ca(2+) at high concentrations. Here we describe a ratiometric low-affinity Ca(2+) sensor of the GFP-aequorin protein (GAP) family optimized for measurements in high-Ca(2+) concentration environments. Transgenic anim...

journal_title：Cell chemical biology

authors： Navas-Navarro P,Rojo-Ruiz J,Rodriguez-Prados M,Ganfornina MD,Looger LL,Alonso MT,García-Sancho J

更新日期：2016-06-23 00:00:00

abstract：:Using light to control cellular processes is one of the attractive areas of research. Here, availability of different, light-responsive caged compounds has played a critical role. In this issue of Cell Chemical Biology, Hövelmann et al. (2016) give us an example of how to design and use caged lipids to guide chemotaxi...

journal_title：Cell chemical biology

authors： Dore TM

更新日期：2016-05-19 00:00:00

abstract：:Dengue virus infects more than 300 million people annually, yet there is no widely protective vaccine or drugs against the virus. Efforts to develop antivirals against classical targets such as the viral protease and polymerase have not yielded drugs that have advanced to the clinic. Here, we show that the allosteric ...

journal_title：Cell chemical biology

authors： Clark MJ,Miduturu C,Schmidt AG,Zhu X,Pitts JD,Wang J,Potisopon S,Zhang J,Wojciechowski A,Hann Chu JJ,Gray NS,Yang PL

更新日期：2016-04-21 00:00:00

abstract：:The discovery of novel small molecules that induce stem cell reprogramming and give efficient access to pluripotent stem cells is of major importance for potential therapeutic applications and may reveal novel insights into the factors controlling pluripotency. Chemical reprogramming of mouse epiblast stem cells (EpiS...

journal_title：Cell chemical biology

authors： Ursu A,Illich DJ,Takemoto Y,Porfetye AT,Zhang M,Brockmeyer A,Janning P,Watanabe N,Osada H,Vetter IR,Ziegler S,Schöler HR,Waldmann H

更新日期：2016-04-21 00:00:00

abstract：:In this issue of Cell Chemical Biology, Ortega et al. (2016) determine the structure of another lantibiotic dehydratase with a tRNA(Glu)-dependent mechanism of modification. Moreover, they identify a common recognition motif involved in leader peptide binding in a number of different peptide-modification enzymes. Thes...

journal_title：Cell chemical biology

authors： Montalbán-López M,Kuipers OP

更新日期：2016-03-17 00:00:00

abstract：:When it comes to lipid diversity, no bacterial genus approaches Mycobacterium. In this issue of Cell Chemical Biology, Burbaud et al. (2016) provide a multi-genic working model for the biosynthesis of trehalose polyphleate (TPP), one of the largest known lipids in mycobacteria. They demonstrate that this lipid is made...

journal_title：Cell chemical biology

authors： Seeliger J,Moody DB

更新日期：2016-02-18 00:00:00

abstract：:The Precision Medicine Initiative aims to use advances in basic and clinical research to develop therapeutics that selectively target and kill cancer cells. Under the same doctrine of precision medicine, there is an equally important need to visualize these diseased cells to enable diagnosis, facilitate surgical resec...

journal_title：Cell chemical biology

authors： Garland M,Yim JJ,Bogyo M

更新日期：2016-01-21 00:00:00